EBiS Characteristic Frequency Using AD5933EBZ for Characterization of DNA Concentration and Purity

Resumen

The estimation of Desoxyribonucleic Acid (DNA) concentrations and purity requires the use of specialized equipment, which tends to be quite expensive and not always available in low-resource laboratories. It is important to develop efforts to seek innovative and inexpensive techniques for such purpose. Electrical Bioimpedance Spectroscopy (EBiS) is a relatively low-cost and easy-to-use technique to characterize the electrical properties of biological analytes. Feature extraction is an important step to explore representative EBiS parameters for analyte characterization. In this study, we analyzed EBiS measurements using the AD5933EBZ board on total DNA samples, aiming to assess whether this device can characterize the concentration and purity of the samples through bioimpedance parameters associated with the characteristic frequency. The results suggest that the reactance parameter values at the characteristic frequencies extracted from EBiS measurements in total DNA samples show less dispersion as DNA concentration and purity increase. The utility of the AD5933EBZ, based on reactance linked to the characteristic frequency, seems viable for detecting DNA concentration and purity ranges with values above 25 ng/µL and 1.8, respectively. Further studies exploring feature extraction in EBiS measurements under controlled DNA concentration and purity conditions are necessary.